rabbit polyclonal primary antibody against tyms  (Proteintech)

Journal: Annals of Translational Medicine

Article Title: Nine hub genes related to the prognosis of HBV-positive hepatocellular carcinoma identified by protein interaction analysis

doi: 10.21037/atm.2020.03.94

Figure Lengend Snippet: Patients’ characteristics (n=77)

Article Snippet: The expression of thymidylate synthase (TYMS) and CDC45 proteins was analyzed in paraffin-embedded HCC tissues by IHC using rabbit polyclonal anti-human antibodies against TYMS and CDC45 (1:100 dilution; Proteintech, Chicago, IL, USA) as previously described ( 16 ).

Techniques: Infection, Expressing

Journal: Annals of Translational Medicine

Article Title: Nine hub genes related to the prognosis of HBV-positive hepatocellular carcinoma identified by protein interaction analysis

doi: 10.21037/atm.2020.03.94

Figure Lengend Snippet: Top 15 hub genes with higher maximal clique centrality value of connectivity

Article Snippet: The expression of thymidylate synthase (TYMS) and CDC45 proteins was analyzed in paraffin-embedded HCC tissues by IHC using rabbit polyclonal anti-human antibodies against TYMS and CDC45 (1:100 dilution; Proteintech, Chicago, IL, USA) as previously described ( 16 ).

Techniques:

Journal: Annals of Translational Medicine

Article Title: Nine hub genes related to the prognosis of HBV-positive hepatocellular carcinoma identified by protein interaction analysis

doi: 10.21037/atm.2020.03.94

Figure Lengend Snippet: Expression of TYMS and CDC45 proteins in patients’ tissues and its association with survival. (A) Representative images of TYMS and CDC45 staining in HBV+ HCC, HBV− HCC, and normal tissues; (B) Kaplan-Meier curves according to TYMS and CDC45 expression levels in HBV+ HCC and HBV− HCC samples from 77 patients; P value was determined by log-rank test. HCC, hepatocellular carcinoma.

Article Snippet: The expression of thymidylate synthase (TYMS) and CDC45 proteins was analyzed in paraffin-embedded HCC tissues by IHC using rabbit polyclonal anti-human antibodies against TYMS and CDC45 (1:100 dilution; Proteintech, Chicago, IL, USA) as previously described ( 16 ).

Techniques: Expressing, Staining

Journal: Molecular medicine reports

Article Title: MEK inhibitor enhanced the antitumor effect of oxaliplatin and 5‑fluorouracil in MEK1 Q56P‑mutant colorectal cancer cells.

doi: 10.3892/mmr.2018.9730

Figure Lengend Snippet: Figure 4. Gene expression induced by U0126 combined with or without oxaliplatin/5‑FU. (A) ERCC1 mRNA levels in SW48 cells were examined by RT‑qPCR following treatment with U0126 and/or oxaliplatin. (B) TYMS mRNA levels in SW48 cells were examined by RT‑qPCR following treatment with U0126 and/or 5‑FU. The graph depicts the fold change in ERCC1/TYMS levels normalized to β‑actin levels. Data are presented as means ± standard deviation. Western blot analysis results of (C) ERCC1 and (D) TYMS protein expression levels in cells treated with U0126 in combination with oxaliplatin or 5‑FU, respectively. **P<0.01 vs. control group; ##P<0.01 vs. oxaliplatin/5‑FU‑treated group. 5‑FU, 5‑fluorouracil; ERCC1, excision repair cross‑complementation group 1; TYMS, thymidylate synthase; RT‑qPCR, reverse transcription‑quantitative polymerase chain reaction.

Article Snippet: Excision repair cross-complementation group 1 (ERCC1) rabbit polyclonal antibody (cat. no. 14586‐1‐AP) and TYMS rabbit polyclonal antibody (cat. no. 15047‐1‐AP) were purchased from ProteinTech Group, Inc. (Wuhan, China).

Techniques: Gene Expression, Standard Deviation, Western Blot, Expressing, Control, Polymerase Chain Reaction